| Solution Information | help | |
| Enzyme: | Procathepsin L | |
| inhibitor: | BDBM59840 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine cathepsin L1 dose response curves for compounds identified as active in one of the following set of experiments entitled, "Inhibitors of Plasmodium falciparum M1- Family Alanyl Aminopeptidase (M1AAP)" (AID 1445), or "Inhibitors of Plasmodium falciparum M17- Family Leucine Aminopeptidase (M17LAP)" (AID 1619). This assay serves as a counterscreen to determine whether compounds are nonselective due to inhibition of cathepsin L1. In this biochemical assay, a commercially available fluorogenic peptide substrate (Z-Leu-Arg-MCA) is incubated with purified recombinant cathepsin L1 protein in the presence of test compounds. Cleavage of the substrate by cathepsin L1 releases the fluorogenic MCA leaving group, leading to an increase in well fluorescence. As designed, compounds that inhibit cathepsin L1 will prevent substrate cleavage and liberation of the fluorescent leaving group, resulting in decreased well fluorescence. Test compounds | |
| If you find an error in this entry please send us an E-mail | ||